VISQUE®InVivo Smart

Preclinical in vivo fluorescent imaging and analysis system

Features

High-sensitivity fluorescence imaging acquisition

Scientific CMOS Camera
Optimized solution for high-end scientific applications
Min. image pixel size: 20um (@7.5x)
High-sensitivity imaging sensor
Quantum Efficiency: 72% at 595nm
Dynamic Range: 87dB
Dark Current: < 10 e-/s/pix @ 30℃
Fast-speed imaging acquisition (Max. 30fps)
Uniformed-quality image with high-speed image acquisition

C57BL/6 mouse image taken 1 hour after NIR fluorescent dye injection through the tail vein.

Nude mouse image taken 2 days after Exosome-NIR dye complex injection through the tail vein.

Representative Detectable Dyes
Representative Detectable Dyes
Imaging – Mode Imaging - Light Excitation / Emission Fluorescent Dyes
GFP Blue Ex : 390nm - 490nm
Em : 500nm - 550nm
GFP / EGFP / Alexa 488 / FITC / QD 525
PE Green Ex : 530nm - 570 nm
Em : 575nm - 640 nm
RFP / DsRed / PE / Alexa 568 / TRITC / QD 585 / QD 605 / QD 625
Cy5.5 Red Ex : 620nm - 650nm
Em : 690nm - 740nm
Cy5.5 / PKE680 / Alexa 680 / Alexa 700 / QD 705
HyperRed Ex : 630nm - 680nm
Em : 690nm - 740nm
ICG NIR Ex : 740nm - 790nm
Em : 810nm - 860nm
ICG / QD 800
Technical Specifications
Technical Specifications
System
Dimension 40 cm x 40 cm x 57 cm
Weight 17 kg
Operating Temperature 10℃ to 27℃
Power 100 – 240 V AC, 50/60 ㎐, max. 0.5 A at 220 V AC
Camera
Sensor scientific CMOS
Resolution (H x V) 1024 x 1024
Pixel Size 6.5 um
Min. Image Pixel Resolution 20 um (7.5x)
Digital Output 14 bit
Maximum Frame Rate 30 fps
Exposure Time 0.013s to 3s
Detection Spectral Range 500 ㎚­ to 860 ㎚
Interface USB 3.0
Lens
Control Zoom / Iris / Focus
Zoom (Field of View, H x V) 15 cm x 15 cm (1x ) ~ 2 cm x 2 cm (7.5x )
Software, CleVueTM
Exclusive File Format *.CIF (CleVue Image File) Saves all information of an image such as a raw image,
analyzed image, ROI information, acquisition information, comments etc.
Supported Image File Format TIFF / Bitmap / JPEG / PNG
Image Merging Merges images of multi-fluorescent dyes
Removal of Autofluorescence Removes autofluorescence or reflection from fluorescent images
Report Mode Displays an analyzed image with color scale bar, analyzed data, acquisition info, comments etc.
Kinetics Analysis • Includes 10 kinds of algorithms, i.e. MTT, BFI, and patented other algorithms to analyze Kinetics
• Dynamics graph, i.e. a plot of pixel intensity over time
• Map with Kinetics values on an image
Excitation Light
Source LED
White Light epi white LED
Emission Filters
Filter Selection Automated Control
Emission Filters 1 included, 8 optional
* Specifications are subject to change without prior notice

Citation Papers

Citation Papers
Title Publication (SCI) Link
Age-related changes in pial arterial structure and blood flow in mice Neurobiol Aging. 2016 Jan;37:161-170 link
Exendin-4 protects hindlimb ischemic injury by inducing angiogenesis Biochem Biophys Res Commun. 2015 Oct 2;465(4):758-63. link
Use of indocyanine green for optical analysis of cortical infarcts in
photothrombotic ischemic brains
J Neurosci Methods. 2015 Jun 15;248:46-50 link
Optical measurement of mouse strain differences in cerebral
blood flow using indocyanine green
J Cereb Blood Flow Metab. 2015 Jun;35(6):912-6 link
Noninvasive Optical Measurement of Cerebral Blood
Flow in Mice Using Molecular Dynamics Analysis of
Indocyanine Green
PLoS One. 2012;7(10):e48383. link
Efficient differentiation of human pluripotent stem cells into
functional CD34 + progenitor cells by combined modulation of the
MEK/ERK and BMP4 signaling pathways
Blood. 2010 Dec 16;116(25):5762-72  link
Unsorted human adipose tissue-derived stem cells promote
angiogenesis and myogenesis in murine ischemic hindlimb model
Microvasc Res. 2010 Dec;80(3):310-6. link
Quantitative Analysis of Peripheral Tissue Perfusion
Using Spatiotemporal Molecular Dynamics
PLoS One. 2009;4(1):e4275. link
Blood flow characteristics of diabetic patients with complications detected by optical measurement BioMedical Engineering OnLine. 2018 17: 25 link



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Catalogue
DOWNLOADInVivoSmart_Catalogue_1807_EN.pdf
DOWNLOADVISQUE InVivo Smart User Manual_V1.1.EN.pdf